Intracellular Localization and Sedimentation Coefficient of DNA Ligase in Oocytes of the Starfish, Asterina pectinifera. (starfish/DNA ligase/germinal vesicle)

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera.

Trypsin was purified from pyloric ceca of the starfish Asterina Pectinifera by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography. Final enzyme preparation was nearly homogeneous in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and its molecular weight was estimated as approximately 28000. Optimum pH and temperature of A. pectinifera trypsin for hydro...

Isolation and characteristics of phospholipase A2 from the pyloric ceca of the starfish Asterina pectinifera

Nuclear distribution of proliferating cell nuclear antigen (PCNA) in fertilized eggs of the starfish Asterina pectinifera.

Previous studies (Nomura et al. (1991) Dev. Biol. 143, 289-296 (1993) Dev. Biol. 159, 288-297) determined the time of DNA replication period (S phase) in starfish eggs fertilized either during or after oocyte maturation. Here proliferating cell nuclear antigen (PCNA) localized within nuclei of starfish eggs was detected with an anti-PCNA human antiserum. Using a confocal laser scanning microsco...

Establishment of embryonic axes in larvae of the starfish, Asterina pectinifera.

In order to clarify the relationships between the first cleavage plane and the embryonic axes, early cleavage pattern of the fertilized eggs of the starfish. Asterina pectinifera was reexamined. It was ascertained that the polar bodies were formed at the site to which the germinal vesicle had closely located before the initiation of the meiotic division, and that the first cleavage plane passed...